Lipid metabolism in human platelets

Lipid metabolism in human platelets.

Altered lipid metabolism in human platelets after primary aggregation.

Thrombin and poly-l-lysine alter the incorporation of acetate, glycerol, and fatty acids into the lipids of washed human platelets. Both aggregating agents decrease the incorporation of acetate into all lipid classes other than free fatty acids. Similarly, glycerol incorporation into complex lipids is impaired by both thrombin and polylysine. Thrombin caused marked depression of the incorporati...

Lysolecithin metabolism by human platelets.

Human platelets incubated with 32p lysolecithin convert this compound to platelet lecithin and the water-soluble product glycerylphosphorylcholine. Lecithin synthesis from lysolecithin by intact platelets has a pH optimum of 9, whereas platelet homogenates show a pH optimum of 7.5 for the same reaction. Conversion of lysolecithin to platelet lecithin takes place by direct acylation of lysolecit...

Calcium-lipid complexes in human platelets.

D URING THE COURSE of a series of investigations into the role of the cell membrane in intercellular cohesion, it became essential to carry out initial studies on a simple model system. Human platelets were chosen for this purpose since they are simple both morphologically and biochemically,’4 their cytoplasmic origin, their lack of nuclei, mitochondria and oxidative enzymes being of distinct a...

Lipid metabolism in human platelets. I. Evidence for a complete fatty acid synthesizing system.

Extracts from human platelets contain the enzymes of de novo fatty acid biosynthesis. The pattern of incorporation of acetate-1-(14)C into fatty acids by intact platelets indicates that these enzymes function in platelets. The level of acetyl-coenzyme A (CoA) carboxylase activity in extracts of platelets from normal subjects is 0.036 +/-0.01 mmumole of malonyl-CoA formed per min per mg of prote...